Thellungiella halophila (salsuginea) workshop, September 6-8 2004, Paris, France - Participants
| NAME | CONTACT | RESEARCH TOPIC | TECHNIQUES AND FACILITIES |
| Anna Amtmann (AA) |
IBLS Bower Building University of Glasgow Glasgow G12 8QQ UK Tel: 44.141.3305393 Fax: 44.141.3304447 E-Mail: a.amtmann@bio.gla.ac.uk |
General:
Ion transport in plant K homeostasis and salt stress. Specific: Characterization of ion transport pathways in roots and leaves of Thellungiella halophila, proteomics of T. halophila. |
Patch clamp; ICP; Micro arrays; 2-D gel electrophoresis, MS; Heterologous expression in yeast and Xenopus. |
| Arie Altman (AAL) |
Institute of Plants
Sciences and Genetics in Agriculture The Hebrew University of Jerusalem P.O. Box 12 Rehovot, 76100 Israel Tel: 972-8-9489477 Fax: 972-8-9489899 E-mail: altman@agri.huji.ac.il |
General:
Molecular and physiological control of abiotic stress tolerance in
plants. Specific: The role boiling-stable chaperone-like proteins and of glycine-betaine in plant response to drought, salinity and osmotic stress, using also transformation with the relevant specific genes in Poplar, Arabidopsis, Thellungiella and Tomato. |
Transformation and tissue culture, cDNA, protein structure-function, in vitro assays of chaperone activity |
| Albert de Boer (AHB) |
Faculty of Earth and
Life Sciences Dept. of Developmental Genetics Vrije Universiteit, De Boelelaan 1085, 1081 HV Amsterdam The Netherlands Tel: 31.20.444.7162 Fax: 31.20.444.7155 E-mail: ahdeboer@bio.vu.nl |
General:
Role of 14-3-3 proteins in plant growth and adaptation to abiotic stress. Specific: Regulation of the H+-ATPase and ion channels in germinating seeds during salt stress and treatment with abscisic acid |
Recombinant protein production; Protein affinity chromatography; RNAi; Mutant screens; Electrophysiology |
| Hans Bohnert (HJB) |
Dept. Plant Biology Dept. Crop Sciences University of Illinois Urbana/Champaign 1201 W. Gregory Drive Urbana, IL 61801 USA Tel: 217-265-5475 Fax: 217-333-5574 E-mail: bohnerth@life.illinois.edu |
General:
Abiotic stress-related transcription programs in yeast, Arabidopsis,
Thellungiella, corn, rice, barley; Eco-arrays - field-grown plants in
elevated CO2 and ozone. Specific: Suppression of stress tolerance Arabidopsis genes in Thellungiella, paralogs of Arabidopsis genes in Thellungiella. Characterization of gene regulation |
cDNA libraries; Expression profiles; Micro arrays; Metabolites by GC-MS; RNAi; Plant transformation; Mutant screens; Physiological techniques; Paralog-ortholog analyses |
| Alain Bouchereau (AB) Laurent Leport |
Osmoadaption and
stress metabolism group UMR CNRS 6026, Bât. 14A Université de Rennes 1 Av. Gal Leclerc, 35042 Rennes France Tel: +33-(0) 2 23 23 69 97 Fax: +33-(0) 2 23 23 69 15 E-mail: alain.bouchereau@univernnes1.fr E-mail: laurent.leport@univennes1.fr |
General:
Biochemical and molecular investigations of abiotic (salinity, drought)
stress metabolism in higher plants. Specific: Significance of compatible solute (i.e. proline, betaine, cyclitols) accumulation and related metabolic adjustments in halophytic and glycophytic plants under osmotic or salt stress. |
Gene expression profiling, Metabolic phenotyping (sugars, amino acids, organic acids, fatty acids, polyamines, betaines); Radio-tracing experiments; Water status measurements; Gas exchange measurements; Plants systems: Canola, A. thaliana, Limonium sp. |
| Ray Bressan (RAB) |
Center for Plant
Environmental Stress Physiology Purdue University 625 Agriculture Mall Drive West Lafayette, IN 47907-2010 USA Tel: 765-494-1336 Fax: 765-494-0391 E-mail: bressan@hort.purdue.edu |
General:
Molecular genetic determinants of abiotic and biotic stress tolerance of
plants and agricultural biotechnology. Specific: Generation of mutant Thellungiella populations by T-DNA activation tagging and screening for genotypes with altered stress responsiveness. |
T-DNA activation tagging; Development of new transformation systems; Tissue culture. |
| Ziyi Cao | Department of
Biology Shandong Normal University 88 East Wenhua Rd. Jinan 250014 P.R. China Tel/Fax: +86-531-6180764 E-mail: caoziyi@beelink.com.cn |
General:
Salt tolerance of Halophytes, Molecular genetics of salt tolerance and
Genetic engineering of salt tolerance. Specific: Genetic polymorphism of Thellungiella halophila ecotypes. T-DNA mutagenesis of Thellungiella. |
Genetics; Proteomics; Gene cloning; transformation; genetic mapping. |
| Wieland Fricke (WF) |
Division of
Biological Sciences University of Paisley Paisley PA1 3BE UK Tel: 44.1418483131 Fax: 44.1418483663 E-mail: fric-bs@wpmail.paisley.ac.uk |
General:
Leaf growth, mineral nutrition and water & solute relations; salinity. Specific: Biophysical control of leaf cell elongation; solute and water transport, and solute compartmentation in leaf tissues. |
Energy-dispersive X-ray (EDX) analysis of pl-samples; Cell pressure probe; Picolitre osmomentry; SEM |
| Marilyn Griffith (MG) |
Department of
Biology University of Waterloo 200 University Ave West Waterloo, Ontario N2L 3G1 Canada Tel: 519-888-4567 (ext. 6441) Fax: 519-746-0614 |
General:
Tolerance to cold and Freezing. Specific: Characterization of freezing tolerance, sensitivity to cold temperature photo-inhibition. |
Chlorophyll fluorescence, freezing experiments; Ice nucleation assays, Assays of freezing tolerance (LT50) |
| Barbara Moffatt (BAM) |
Department of
Biology University of Waterloo 200 University Ave. West Waterloo, Ontario N2L 3G1 Canada Tel: 519-888-4567 (ext. 2517) Fax: 519-746-0614 E-mail: moffatt@sciborg.uwaterloo.ca |
General:
Tolerance to cold and freezing. Molecular biology of methyl cycling. Specific: ESTs and micro arrays of genes induced by high salt, cold and drought in the Thellungiella salsuginea |
Micro arrays; Cloning; Transformation |
| Jose M. Pardo (JMP) |
Instituto de
Recursos Naturales y Agrobiologia Consejo Superior de Investigaciones Cientificas Reina Mercedes, 10 Sevilla 41012 Spain Tel: (+34) 954 62 47 11 Fax: (+34) 954 62 40 02 E-mail: Pardo@cica.es |
General:
Salinity tolerance of plants. Sodium and potassium transport. Specific: Genetic determinates of environmental stress tolerance of Thellungiella halophila |
Cation transporters; Heterologous expressions in yeast, ICP. |
| Arnould Savouré (AS) |
Cellular and
Molecular Plant Physiology UMR 7632 CNRS, Case 156 Université Pierre & Marie Curie 4 Place Jussieu, 75252 Paris 5 France Tel/Fax: +33-(0)1 44 27 26 72 E-mail: savoure@ccr.jussieu.fr |
General:
Molecular mechanisms underlying plant water stress adaptation. Specific: Characterization of the signaling pathways involved in the regulation of pectin methyl esterase on cell wall properties and of proline metabolism upon water stress. |
Macrorrays; Enzyme activities; Protein gel electrophorsis; Lipid analysis; Heterologous expression |
| Erik Souer (ES) |
Dep. of Ecology and
Physiology of Plants Vrije Universiteit, De Boelelaan 1085, 1081 HV Amsterdam The Netherlands Tel: +31-20-4447050 Fax: +31-20-4447123 E-mail: erik.souer@falw.vu.nl |
General:
Molecular responses to salt and dehydration stress. Specific: Gene Expression profiling and T-DNA tagging in Thellungiella halophila |
cDNA AFLP; Plant transformation |
| Teruaki Taji (TT) |
Shinozaki Lab Riken 3-1-1 Koyadai Tsukuba, Ibaraki 305-0074 Japan Tel: 81-29-836-4359 Fax: 81-29-836-9060 E-mail: taji@rtc.riken.jp |
General:
Signaling pathways involved in plant responses to environmental stresses. Specific: Transcriptomics of Thellungiella halophila |
|
| Elizabeth Weretilnyk (EAW) |
Department of
Biology McMaster University Hamilton ON, L8S 4K1 Canada Tel: 905.525.9140 (Ext. 24573) Fax: 905.522.6066 E-mail: weretil@mcmail.mcmaster.ca |
General:
Biochemical and molecular genetic analysis of plant metabolic
adaptations toward environmental stress. Specific: Metabolomics of Thellungiella salsuginea in response to high salt, cold and drought. |
Mass spectrometry; Water potential; Physiological techniques |
| Jian-Kang Zhu (JKZ) |
Department of Botany
and Plant Sciences Institute for Integrative Genome Biology University of California 2150 Batchelor Hall Riverside CA, 92521 USA Tel: 1-951-827-7117 Fax: 1-951-827-7115 E-mail: jian-kang.zhu@ucr.edu |
General:
Signaling pathways involved in plant responses to environmental stresses.
Identification of key genes for modifying the responses of crops to
environmental stresses. Specific: Screening and analysis of stress-oversensitive Arabidopsis thaliana and Thellungiella halophila mutants. |
Genetics; Protein biochemistry; Proteomics; Promoter-reporter constructs; Transcriptional and post transcriptional gene silencing |
Specific topic: Thellungiella
Inan G et al. (2003) Salt cress (Thellungiella halophila): A halophyte
and cryophyte Arabidopsis relative model system (ARMS) and its
applicability to melecular genetic analyses of growth and development
extremophiles. (RAB, HJB, JKZ).
Volkov V et al. (2004) Thellungiella halophila, a salt-tolerant
relative to Arabidopsis thaliana, possesses effective mechanisms to
discriminate between potassium and sodium. Plant, Cell Environ 27,
1-14 (AA, WF).
Teusink RS et al. (2002) Cuticular waxes on Arabidopsis thaliana
close relatives Thellungiella halophila and Thellungiella parvula.
Int J Plant Sci 163: 309-315 (RAB).
Bressan RA et al. (2001) Learning from the Arabidopsis Experience.
The next gene search paradigm. Plants Physiol 127, 1354-1360 (RAB,
HJB, JKZ).
General topic: Abiotic stress
De Boer AH, Volkov V (2003) Logistics of water and salt transport through the
plant: structure and functioning of the xylem. Plant Cell Environ.
26 (1): 87-101 (AHB).
Maathuis FJM et al. (2003) Transcriptome analysis of root transporters
reveals participation of multiple gene families in response to cation stress.
Plant J 35 (6): 675-692 (AA).
Shi HZ et al. (2003) Over-expression of a plasma membrane Na+/H+ antiporter
gene improves salt tolerance in Arabidopsis thaliana. Nature
Biotech 21 (1): 81-85 (JKZ).
Golldack D et al. (2002) Characterization of a HKT-type transporter in rice
as a general alkali cation transporter. Plant J. 31 (4): 529-542
(HJB).
Fricke W (2002) Biophysical limitation of cell elongation in cereal leaves.
Ann Bot. 90 (2) 157-167 (WF).
Fricke W, Peters WS (2002) The biophysics of leaf growth in salt-stressed
barley. A study at the cell level. Plant Physiol. 129 (1): 374-388
(WF)
Gong ZZ et al. (2002) RNA helicase-like protein as an early regulator of
transcription factors for plant chilling and freezing tolerance. PNAS.
99 (17): 11507-11512 (JKZ).
Hasegawa PM et al. (2000) Plant cellular and molecular responses to high
salinity. Ann Rev Plant Phys. 51: 463-499 (HJB, RAB, JKZ).
Koiwa H et al. (2002) C-terminal domain phosphatase-like family members (AtCPLs)
differentially regulate Arabidopsis thaliana abiotic stress signaling,
growth and development. PNAS. 99 (16): 10893-10898 (RAB).
Su H et al. (2002) Zhu JH et al. (2002) OSM1/SYP61: A syntaxin protein in
Arabidopsis controls Abscisic acid-mediated and non-abscisic acid-mediated
responses to abiotic stress. Plant Cell. 14 (12): 3009-3028 (RAB).
Amtmann A et al. (2001) The wheat cDNA LCT1 generates hypersensitivity to
sodium in salt-sensitive yeast strain. Plant Physiol. 126 (3)
1061-1071 (AA).
Bunney TD et al. (2001) 14-3-3 protein is a regulator of the mitochondrial
and chloroplast ATP synthase. PNAS. 98 (7): 4249-4254 (AHB).
Kawasaki S et al. (2001) Gene expression profiles during the initial phase
of salt stress in rice. Plant Cell. 13 (4): 889-905 (HJB).
Weretilnyk EA et al. (2001) Maintaining methylation activities during salt
stress. The involvement of adenosine kinase. Plant Physiol. 125
(2): 856-865 (EAW).
Yu XM et al. (2001) Ethylene induces antifreeze activity in winter rye
leaves. Plant Physiol. 126 (3): 1232-1240 (MG).
Yeh S et al. (2000) Chitinase genes responsive to cold encode antifreeze
proteins in winter cereals. Plant Physiol. 124 (3): 1251-1263
(BAM, MG).
Savoure A et al. (1999) NaCl and CuSO4 treatments trigger distinct oxidative
defense mechanisms in Nicotiana plumbaginifolia L. Plant Cell
Environ. 22: 387-396 (AS).
van Houwelingen A et al. (1999) Epigenetic interactions among three dTph1
transposons in two homologous chromosomes activate a new excision-repair
mechanism in petunia. Plant Cell.11 (7): 1319-1336
(ES).
Savoure A et al. (1998) Abscisic acid-independent and abscisic
acid-dependent regulation of proline biosynthesis following cold and osmotic
stresses in Arabidopsis thaliana. Mol Gen Genetics. 254
(1): 104-109 (AS).
Wang, W.G. et al. (2002) cDNA cloning, expression and characterization of
SP1, an oligomeric water-stress responsive boiling stable protein. Plant
Physiol. 130: 865-875 (AAL).
Wang, W. et al. (2003) Crystallization and preliminary X-ray
crystallographic analysis of SP1, a novel chaperone-like protein. Acta
Cryst. D59: 512-514 (AAL).
Wang, W. et al. (2003) Plant responses to drought, salinity and extreme
temperatures: towards genetic engineering for stress tolerance. Planta
218: 1-14 (AAL).